Target Interacting Protein Information

Content Navigation (  All   None )                                      
Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T10822 Target Info
Target Name Cyclin-dependent kinase 8 (CDK8)
Synonyms Protein kinase K35; Mediator of RNA polymerase II transcription subunit CDK8; Mediator complex subunit CDK8; Cell division protein kinase 8
Target Type Clinical trial Target
Gene Name CDK8
Biochemical Class Kinase
UniProt ID
CDK8_HUMAN 
Target Interacting Proteins  Top
Cell cycle related proteins [+] 1 Cell cycle related proteins +
Interacting Protein Name Cyclin-C (CCNC) Interacting Protein Interacting Protein Info
UniProt ID
CCNC_HUMAN 
Synonyms CycC
Evidence Score (E-score) 12 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Affinity Capture-Western [5]
6 Affinity Capture-Western [6]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [8]
9 Co-fractionation [9]
10 Co-purification [10]
11 Reconstituted Complex [6]
12 Reconstituted Complex [11]
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Protein Name RNA polymerase II subunit A C-terminal domain phosphatase (CTDP1) Interacting Protein Interacting Protein Info
UniProt ID
CTDP1_HUMAN 
Synonyms CCFDN; FCP1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Biochemical Activity [12]
Kinases [+] 1 Kinases +
Interacting Protein Name DNA-directed RNA polymerase II subunit RPB1 (POLR2A) Interacting Protein Interacting Protein Info
UniProt ID
RPB1_HUMAN 
Synonyms POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Biochemical Activity [13]
3 Biochemical Activity [11]
4 Biochemical Activity [14]
5 Biochemical Activity [15]
6 Co-fractionation [9]
Mediator complexes [+] 18 Mediator complexes +
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 6 (MED6) Interacting Protein Interacting Protein Info
UniProt ID
MED6_HUMAN 
Synonyms ARC33; NY.REN.28
Evidence Score (E-score) 12 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [10]
7 Affinity Capture-Western [3]
8 Affinity Capture-Western [5]
9 Affinity Capture-Western [7]
10 Affinity Capture-Western [19]
11 Co-fractionation [20]
12 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 12 (MED12) Interacting Protein Interacting Protein Info
UniProt ID
MED12_HUMAN 
Synonyms ARC240; CAGH45; FGS1; HOPA; MED12S; OHDOX; OKS; OPA1; TNRC11; TRAP230
Evidence Score (E-score) 11 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [21]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [5]
9 Affinity Capture-Western [22]
10 Affinity Capture-Western [23]
11 Co-purification [24]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 24 (MED24) Interacting Protein Interacting Protein Info
UniProt ID
MED24_HUMAN 
Synonyms ARC100; CRSP100; CRSP4; DRIP100; THRAP4; TRAP100
Evidence Score (E-score) 11 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [10]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [5]
9 Affinity Capture-Western [22]
10 Co-fractionation [20]
11 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 1 (MED1) Interacting Protein Interacting Protein Info
UniProt ID
MED1_HUMAN 
Synonyms CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
Evidence Score (E-score) 10 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [18]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [2]
5 Affinity Capture-MS [10]
6 Affinity Capture-MS [21]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [5]
9 Affinity Capture-Western [22]
10 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 14 (MED14) Interacting Protein Interacting Protein Info
UniProt ID
MED14_HUMAN 
Synonyms CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; RGR1; TRAP170
Evidence Score (E-score) 10 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [10]
7 Affinity Capture-MS [21]
8 Affinity Capture-Western [3]
9 Affinity Capture-Western [7]
10 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 13 (MED13) Interacting Protein Interacting Protein Info
UniProt ID
MED13_HUMAN 
Synonyms ARC250; DRIP250; HSPC221; THRAP1; TRAP240
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [21]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [5]
9 Affinity Capture-Western [22]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 17 (MED17) Interacting Protein Interacting Protein Info
UniProt ID
MED17_HUMAN 
Synonyms CRSP6; CRSP77; DRIP80; TRAP80
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [21]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [5]
9 Affinity Capture-Western [23]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 23 (MED23) Interacting Protein Interacting Protein Info
UniProt ID
MED23_HUMAN 
Synonyms ARC130; CRSP130; CRSP133; CRSP3; DRIP130; MRT18; SUR.2; SUR2
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [21]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-Western [3]
7 Affinity Capture-Western [5]
8 Affinity Capture-Western [8]
9 Affinity Capture-Western [22]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 10 (MED10) Interacting Protein Interacting Protein Info
UniProt ID
MED10_HUMAN 
Synonyms NUT2; TRG20
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [10]
7 Affinity Capture-Western [5]
8 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 16 (MED16) Interacting Protein Interacting Protein Info
UniProt ID
MED16_HUMAN 
Synonyms DRIP92; THRAP5; TRAP95
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-MS [21]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [5]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 15 (MED15) Interacting Protein Interacting Protein Info
UniProt ID
MED15_HUMAN 
Synonyms ARC105; CAG7A; CTG7A; PCQAP; TIG.1; TIG1; TNRC7
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [21]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
6 Affinity Capture-Western [23]
7 Co-purification [24]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 21 (MED21) Interacting Protein Interacting Protein Info
UniProt ID
MED21_HUMAN 
Synonyms SRB7; SURB7; hSrb7
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [1]
3 Affinity Capture-MS [2]
4 Affinity Capture-Western [7]
5 Affinity Capture-Western [5]
6 Co-fractionation [9]
7 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 20 (MED20) Interacting Protein Interacting Protein Info
UniProt ID
MED20_HUMAN 
Synonyms PRO0213; TRFP
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [17]
2 Affinity Capture-MS [18]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [2]
5 Affinity Capture-Western [7]
6 Affinity Capture-Western [5]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 22 (MED22) Interacting Protein Interacting Protein Info
UniProt ID
MED22_HUMAN 
Synonyms MED24; SURF5; surf.5
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [21]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 31 (MED31) Interacting Protein Interacting Protein Info
UniProt ID
MED31_HUMAN 
Synonyms 3110004H13Rik; Soh1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-MS [1]
3 Affinity Capture-MS [2]
4 Affinity Capture-MS [10]
5 Co-purification [10]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 8 (MED8) Interacting Protein Interacting Protein Info
UniProt ID
MED8_HUMAN 
Synonyms ARC32
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [17]
2 Affinity Capture-MS [18]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [2]
5 Co-fractionation [20]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 9 (MED9) Interacting Protein Interacting Protein Info
UniProt ID
MED9_HUMAN 
Synonyms MED25
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [21]
2 Affinity Capture-MS [17]
3 Affinity Capture-MS [18]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [2]
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 7 (MED7) Interacting Protein Interacting Protein Info
UniProt ID
MED7_HUMAN 
Synonyms ARC34; CRSP33; CRSP9
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [2]
2 Affinity Capture-Western [7]
3 Affinity Capture-Western [5]
4 Affinity Capture-Western [8]
Transcription regulators [+] 1 Transcription regulators +
Interacting Target Name Sterol regulatory element binding protein-1 (SREBF1) Literature-reported Target Interacting Protein Info
UniProt ID
SRBP1_HUMAN 
Synonyms Sterol regulatory element-binding transcription factor 1; Sterol regulatory element-binding protein 1; SREBP-1; SREBP1; SREBP 1; Class D basic helix-loop-helix protein 1; bHLHd1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Reconstituted Complex [26]
Other proteins [+] 1 Other proteins +
Interacting Target Name Poly [ADP-ribose] polymerase 1 (PARP1) Successful Target Interacting Protein Info
UniProt ID
PARP1_HUMAN 
Synonyms Protein poly-ADP-ribosyltransferase PARP1; PPOL; Poly[ADP-ribose] synthetase-1; Poly[ADP-ribose] synthase 1; Poly(ADP-ribose)polymerase-1; PARP-1; NAD(+)Poly [ADP-ribose] polymerase-1 ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase 1; DNA ADP-ribosyltransferase PARP1; ARTD1; ADPRT 1; ADPRT; ADP-ribosyltransferase diphtheria toxin-like 1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [19]
2 Reconstituted Complex [27]
References  Top
REF 1 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 2 The protein interaction landscape of the human CMGC kinase group. Cell Rep. 2013 Apr 25;3(4):1306-20.
REF 3 Characterization of mediator complexes from HeLa cell nuclear extract. Mol Cell Biol. 2001 Jul;21(14):4604-13.
REF 4 Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex. Cell. 1997 May 2;89(3):357-64.
REF 5 The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2642-7.
REF 6 Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C. Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8871-5.
REF 7 The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs. Mol Cell. 2000 Apr;5(4):753-60.
REF 8 Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein. Nature. 1999 May 20;399(6733):276-9.
REF 9 A human RNA polymerase II complex containing factors that modify chromatin structure. Mol Cell Biol. 1998 Sep;18(9):5355-63.
REF 10 A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. Mol Cell. 1999 Jan;3(1):97-108.
REF 11 A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1. Biol Proced Online. 2004;6:163-172.
REF 12 Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways. Cell. 2009 Nov 13;139(4):757-69.
REF 13 Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II. Eur J Biochem. 2004 Mar;271(5):1004-14.
REF 14 Retroviral cyclin enhances cyclin-dependent kinase-8 activity. J Virol. 2012 May;86(10):5742-51.
REF 15 Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences. J Biol Chem. 2001 Apr 6;276(14):10913-20.
REF 16 Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. Mol Cell. 1999 Mar;3(3):361-70.
REF 17 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 18 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-440.
REF 19 PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator. Mol Cell. 2005 Apr 1;18(1):83-96.
REF 20 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 21 CDK8 is a positive regulator of transcriptional elongation within the serum response network. Nat Struct Mol Biol. 2010 Feb;17(2):194-201.
REF 22 The SCF-Fbw7 ubiquitin ligase degrades MED13 and MED13L and regulates CDK8 module association with Mediator. Genes Dev. 2013 Jan 15;27(2):151-6.
REF 23 Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes. J Biol Chem. 2013 Jul 19;288(29):20955-65.
REF 24 Mediator and cohesin connect gene expression and chromatin architecture. Nature. 2010 Sep 23;467(7314):430-5.
REF 25 Structure, function, and activator-induced conformations of the CRSP coactivator. Science. 2002 Feb 8;295(5557):1058-62.
REF 26 An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. Nature. 2006 Aug 10;442(7103):700-4.
REF 27 Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription. J Biol Chem. 2005 Dec 9;280(49):40450-64.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.