Binding Site Information of Target
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T17048 | Target Info | |||
| Target Name | DNA topoisomerase II alpha (TOP2A) | ||||
| Synonyms | DNA topoisomerase II, alpha isozyme; DNA topoisomerase 2alpha; DNA topoisomerase 2-alpha | ||||
| Target Type | Clinical trial Target | ||||
| Gene Name | TOP2A | ||||
| Biochemical Class | Topoisomerase | ||||
| UniProt ID | |||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| Ligand Name: Etoposide | Ligand Info | |||||
| Structure Description | Cryo-EM structure of the Human topoisomerase II alpha DNA-binding/cleavage domain in State 1 | PDB:6ZY5 | ||||
| Method | Electron microscopy | Resolution | 3.60 Å | Mutation | No | [1] |
| PDB Sequence |
NRIKGIPKLD
442 DANDAGGRNS452 TECTLILTEG462 DSAKTLAVSG472 LGVVGRDKYG482 VFPLRGKILN 492 VREASHKQIM502 ENAEINNIIK512 IVGLQYKKNY522 EDEDSLKTLR532 YGKIMIMTDQ 542 DQDGSHIKGL552 LINFIHHNWP562 SLLRHRFLEE572 FITPIVKVSK582 NKQEMAFYSL 592 PEFEEWKSST602 PNHKKWKVKY612 YKGLGTSTSK622 EAKEYFADMK632 RHRIQFKYSG 642 PEDDAAISLA652 FSKKQIDDRK662 EWLTNFMEDR672 RQRKLLGLPE682 DYLYGQTTTY 692 LTYNDFINKE702 LILFSNSDNE712 RSIPSMVDGL722 KPGQRKVLFT732 CFKRNDKREV 742 KVAQLAGSVA752 EMSSYHHGEM762 SLMMTIINLA772 QNFVGSNNLN782 LLQPIGQFGT 792 RLHGGKDSAS802 PRYIFTMLSS812 LARLLFPPKD822 DHTLKFLYDD832 NQRVEPEWYI 842 PIIPMVLING852 AEGIGTGWSC862 KIPNFDVREI872 VNNIRRLMDG882 EEPLPMLPSY 892 KNFKGTIEEL902 APNQYVISGE912 VAILNSTTIE922 ISELPVRTWT932 QTYKEQVLEP 942 MLNGTEKTPP952 LITDYREYHT962 DTTVKFVVKM972 TEEKLAEAER982 VGLHKVFKLQ 992 TSLTCNSMVL1002 FDHVGCLKKY1012 DTVLDILRDF1022 FELRLKYYGL1032 RKEWLLGMLG 1042 AESAKLNNQA1052 RFILEKIDGK1062 IIIENKPKKE1072 LIKVLIQRGY1082 DSDPVKAWKE 1092 AQQKVSGPTF1125 NYLLDMPLWY1135 LTKEKKDELC1145 RLRNEKEQEL1155 DTLKRKSPSD 1165 LWKEDLATFI1175 EELEAVEAKE1185 KQDEQVGLPG1195 KGGKAKGKKT1205 QMAEVLPSPR 1215
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| Ligand Name: AMP-PNP | Ligand Info | |||||
| Structure Description | Human Topo IIa ATPase/AMP-PNP | PDB:1ZXM | ||||
| Method | X-ray diffraction | Resolution | 1.87 Å | Mutation | No | [2] |
| PDB Sequence |
SVERIYQKKT
38 QLEHILLRPD48 TYIGSVELVT58 QQMWVYDEDV68 GINYREVTFV78 PGLYKIFDEI 88 LVNAADNKQR98 DPKMSCIRVT108 IDPENNLISI118 WNNGKGIPVV128 EHKVEKMYVP 138 ALIFGQLLTS148 SNYDDDEKKV158 TGGRNGYGAK168 LCNIFSTKFT178 VETASREYKK 188 MFKQTWMDNM198 GRAGEMELKP208 FNGEDYTCIT218 FQPDLSKFKM228 QSLDKDIVAL 238 MVRRAYDIAG248 STKDVKVFLN258 GNKLPVKGFR268 SYVDMYLKDK278 LDETGNSLKV 288 IHEQVNHRWE298 VCLTMSEKGF308 QQISFVNSIA318 TSKGGRHVDY328 VADQIVTKLV 338 DVVKKKNAVK352 AHQVKNHMWI362 FVNALIENPT372 FDSQTKENMT382 LQPKSFGSTC 392 QLSEKFIKAA402 IGC
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GLU87
3.581
ASN91
2.909
ALA92
4.058
ASP94
4.395
ASN95
3.159
ARG98
3.681
ASN120
2.824
LYS123
4.280
GLY124
4.999
ILE125
3.404
ILE141
3.433
PHE142
3.601
THR147
3.466
SER148
2.758
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| Ligand Name: adenosine diphosphate | Ligand Info | |||||
| Structure Description | Human DNA topoisomerase IIa ATPase/ADP | PDB:1ZXN | ||||
| Method | X-ray diffraction | Resolution | 2.51 Å | Mutation | No | [2] |
| PDB Sequence |
SVERIYQKKT
38 QLEHILLRPD48 TYIGSVELVT58 QQMWVYDEDV68 GINYREVTFV78 PGLYKIFDEI 88 LVNAADNKQR98 DPKMSCIRVT108 IDPENNLISI118 WNNGKGIPVV128 EHKVEKMYVP 138 ALIFGQLLTS148 SNYDDDEKKV158 TGGRNGYGAK168 LCNIFSTKFT178 VETASREYKK 188 MFKQTWMDNM198 GRAGEMELKP208 FNGEDYTCIT218 FQPDLSKFKM228 QSLDKDIVAL 238 MVRRAYDIAG248 STKDVKVFLN258 GNKLPVKGFR268 SYVDMYLKVI289 HEQVNHRWEV 299 CLTMSEKGFQ309 QISFVNSIAT319 SKGGRHVDYV329 ADQIVTKLVH354 QVKNHMWIFV 364 NALIENPTFD374 SQTKENMTLQ384 PKSFGSTCQL394 SEKFIKAAIG404 IVESILNWVK 416 FKAQVQ
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Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:87 or .A:91 or .A:92 or .A:94 or .A:95 or .A:98 or .A:120 or .A:123 or .A:125 or .A:141 or .A:142 or .A:147 or .A:148 or .A:149 or .A:150 or .A:161 or .A:162 or .A:163 or .A:164 or .A:165 or .A:166 or .A:167 or .A:168 or .A:215; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
GLU87
4.988
ASN91
2.836
ALA92
3.948
ASP94
4.434
ASN95
3.224
ARG98
3.568
ASN120
2.975
LYS123
4.730
ILE125
3.504
ILE141
3.228
PHE142
3.709
THR147
4.130
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Structural basis for allosteric regulation of Human Topoisomerase IIAlpha. Nat Commun. 2021 May 20;12(1):2962. | ||||
| REF 2 | Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase. J Biol Chem. 2005 Nov 4;280(44):37041-7. | ||||
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