Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T08391 Target Info
Target Name Janus kinase 2 (JAK-2)
Synonyms Tyrosine-protein kinase JAK2
Target Type Successful Target
Gene Name JAK2
Biochemical Class Kinase
UniProt ID
JAK2_HUMAN 
Target Interacting Proteins  Top
Adapter proteins [+] 1 Adapter proteins +
Interacting Protein Name Growth factor receptor-bound protein 2 (GRB2) Interacting Protein Interacting Protein Info
UniProt ID
GRB2_HUMAN 
Synonyms SH2/SH3 adapter GRB2; Protein Ash; Growth factor receptor-bound protein 2; ASH; Adapter protein GRB2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Reconstituted Complex [2]
Cytokine receptors [+] 2 Cytokine receptors +
Interacting Target Name Erythropoietin Receptor (EPOR) Successful Target Interacting Protein Info
UniProt ID
EPOR_HUMAN 
Synonyms Full-length form; EPO-R
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Reconstituted Complex [4]
Interacting Target Name Growth hormone receptor (GHR) Successful Target Interacting Protein Info
UniProt ID
GHR_HUMAN 
Synonyms Somatotropin receptor; Serum bindingprotein; Growth hormone receptor; GH receptor
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [5]
2 Co-fractionation [6]
Ester hydrolases [+] 2 Ester hydrolases +
Interacting Target Name Protein-tyrosine phosphatase SHP-2 (PTPN11) Clinical trial Target Interacting Protein Info
UniProt ID
PTN11_HUMAN 
Synonyms Tyrosine-protein phosphatase non-receptor type 11; SH-PTP3; SH-PTP2; SHPTP2; SHP-2; SHP2; PTP-2C; PTP2C; PTP-1D; Protein-tyrosine phosphatase SHP2; Protein-tyrosine phosphatase 2C; Protein-tyrosine phosphatase 1D
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Biochemical Activity [8]
3 Biochemical Activity [9]
Interacting Target Name Protein-tyrosine phosphatase 1B (PTP1B) Clinical trial Target Interacting Protein Info
UniProt ID
PTN1_HUMAN 
Synonyms Tyrosine-protein phosphatase non-receptor type 1; PTP-1B
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Biochemical Activity [11]
Kinases [+] 1 Kinases +
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Biochemical Activity [13]
3 Protein-peptide [14]
One-carbon transferases [+] 1 One-carbon transferases +
Interacting Target Name Protein arginine methyltransferase 5 (PRMT5) Clinical trial Target Interacting Protein Info
UniProt ID
ANM5_HUMAN 
Synonyms SKB1Hs; SKB1 homolog; SKB1; Shk1 kinase-binding protein 1 homolog; Protein arginine N-methyltransferase 5; JBP1; Jak-binding protein 1; IBP72; HRMT1L5; Histone-arginine N-methyltransferase PRMT5; 72 kDa ICln-binding protein
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [15]
2 Biochemical Activity [15]
3 Yeast two hybrid [16]
Transcription regulators [+] 4 Transcription regulators +
Interacting Target Name Signal transducer and activator of transcription 3 (STAT3) Successful Target Interacting Protein Info
UniProt ID
STAT3_HUMAN 
Synonyms Transcription factor STAT3; Stat3; Signal transducer and activator of transcription 3; APRF; Acute-phase response factor
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [17]
2 Affinity Capture-Western [18]
3 Biochemical Activity [13]
4 Biochemical Activity [19]
Interacting Protein Name Signal transducer and activator of transcription 5A (STA5A) Interacting Protein Interacting Protein Info
UniProt ID
STA5A_HUMAN 
Synonyms MGF; STAT5
Evidence Score (E-score) 3 Experimental Evidences +
1 Biochemical Activity [20]
2 Reconstituted Complex [21]
3 Yeast two hybrid [21]
Interacting Target Name Signal transducer and activator of transcription 1 (STAT1) Patented-recorded Target Interacting Protein Info
UniProt ID
STAT1_HUMAN 
Synonyms Transcription factor ISGF-3 components p91/p84; STAT-1; Signal transducers and activators of transcription factor; Signal transducer and activator of transcription 1-alpha/beta; P91
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Co-localization [23]
Interacting Protein Name Signal transducer and activator of transcription 5B (STA5B) Interacting Protein Interacting Protein Info
UniProt ID
STA5B_HUMAN 
Synonyms STAT5
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [24]
2 Yeast two hybrid [21]
Other proteins [+] 5 Other proteins +
Interacting Target Name Suppressor of cytokine signaling 1 (SOCS1) Literature-reported Target Interacting Protein Info
UniProt ID
SOCS1_HUMAN 
Synonyms TIP-3; TIP3; Tec-interacting protein 3; STAT-induced STAT inhibitor 1; STAT induced STAT inhibitor 1; SSI-1; SSI1; SOCS-1; JAK-binding protein; JAB
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-Western [25]
2 Affinity Capture-Western [26]
3 Affinity Capture-Western [27]
4 Affinity Capture-Western [28]
5 Affinity Capture-Western [29]
6 Reconstituted Complex [30]
7 Yeast two hybrid [31]
8 Yeast two hybrid [32]
Interacting Target Name Suppressor of cytokine signaling 3 (SOCS3) Literature-reported Target Interacting Protein Info
UniProt ID
SOCS3_HUMAN 
Synonyms STAT-induced STAT inhibitor 3; STAT induced STAT inhibitor 3; SSI-3; SSI3; SOCS-3; Cytokine-inducible SH2 protein 3; CIS-3; CIS3
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-Western [33]
2 Affinity Capture-Western [34]
3 Affinity Capture-Western [29]
4 Reconstituted Complex [35]
5 Reconstituted Complex [30]
6 Reconstituted Complex [24]
7 Yeast two hybrid [35]
Interacting Protein Name Proto-oncogene vav (VAV1) Interacting Protein Interacting Protein Info
UniProt ID
VAV_HUMAN 
Synonyms VAV
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [36]
2 Affinity Capture-Western [37]
3 Biochemical Activity [36]
Interacting Target Name Tumor necrosis factor receptor type I (TNF-R1) Clinical trial Target Interacting Protein Info
UniProt ID
TNR1A_HUMAN 
Synonyms Tumor necrosis factor-binding protein 1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-RI; TNFR-I; TNFR1; TNFAR; TBPI; p60; p55; CD120a
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [38]
2 Reconstituted Complex [38]
Interacting Target Name Insulin receptor substrate-1 (IRS1) Clinical trial Target Interacting Protein Info
UniProt ID
IRS1_HUMAN 
Synonyms IRS-1; Insulin receptor substrate 1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [39]
2 Biochemical Activity [40]
References  Top
REF 1 Oncostatin M induces association of Grb2 with Janus kinase JAK2 in multiple myeloma cells. J Exp Med. 1995 Dec 1;182(6):1801-6.
REF 2 Involvement of Janus kinases in the insulin signaling pathway. Eur J Biochem. 1995 Dec 1;234(2):656-60.
REF 3 The N-terminal domain of Janus kinase 2 is required for Golgi processing and cell surface expression of erythropoietin receptor. Mol Cell. 2001 Dec;8(6):1327-38.
REF 4 JAK2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin. Cell. 1993 Jul 30;74(2):227-36.
REF 5 Regions of the JAK2 tyrosine kinase required for coupling to the growth hormone receptor. J Biol Chem. 1995 Jun 16;270(24):14776-85.
REF 6 Multimeric growth hormone receptor complexes serve as signaling platforms. J Biol Chem. 2014 Jan 3;289(1):65-73.
REF 7 SHPTP2 serves adapter protein linking between Janus kinase 2 and insulin receptor substrates. Biochem Biophys Res Commun. 1996 Nov 1;228(1):122-7.
REF 8 Molecular characterization of specific interactions between SHP-2 phosphatase and JAK tyrosine kinases. J Biol Chem. 1997 Jan 10;272(2):1032-7.
REF 9 Tyrosine 425 within the activated erythropoietin receptor binds Syp, reduces the erythropoietin required for Syp tyrosine phosphorylation, and promotes mitogenesis. Blood. 1996 Jun 1;87(11):4495-501.
REF 10 TYK2 and JAK2 are substrates of protein-tyrosine phosphatase 1B. J Biol Chem. 2001 Dec 21;276(51):47771-4.
REF 11 Mechanism of protein tyrosine phosphatase 1B-mediated inhibition of leptin signalling. J Mol Endocrinol. 2005 Apr;34(2):339-51.
REF 12 ErbB receptor-induced activation of stat transcription factors is mediated by Src tyrosine kinases. J Biol Chem. 1999 Jun 11;274(24):17209-18.
REF 13 Blockade of glioma proliferation through allosteric inhibition of JAK2. Sci Signal. 2013 Jul 9;6(283):ra55.
REF 14 A quantitative protein interaction network for the ErbB receptors using protein microarrays. Nature. 2006 Jan 12;439(7073):168-74.
REF 15 JAK2V617F-mediated phosphorylation of PRMT5 downregulates its methyltransferase activity and promotes myeloproliferation. Cancer Cell. 2011 Feb 15;19(2):283-94.
REF 16 The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity. J Biol Chem. 1999 Oct 29;274(44):31531-42.
REF 17 Interaction of growth hormone-activated STATs with SH2-containing phosphotyrosine phosphatase SHP-1 and nuclear JAK2 tyrosine kinase. J Biol Chem. 1997 Jul 11;272(28):17694-702.
REF 18 EGFR-induced cell migration is mediated predominantly by the JAK-STAT pathway in primary esophageal keratinocytes. Am J Physiol Gastrointest Liver Physiol. 2004 Dec;287(6):G1227-37.
REF 19 Overexpression of the oncostatin-M receptor in cervical squamous cell carcinoma is associated with epithelial-mesenchymal transition and poor overall survival. Br J Cancer. 2016 Jul 12;115(2):212-22.
REF 20 Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription. EMBO J. 1994 Sep 15;13(18):4361-9.
REF 21 An alternative pathway for STAT activation that is mediated by the direct interaction between JAK and STAT. Oncogene. 1997 Feb 20;14(7):751-61.
REF 22 Stat1 mediates an auto-regulation of hsp90beta gene in heat shock response. Cell Signal. 2010 Aug;22(8):1206-13.
REF 23 Jak/STAT signaling pathway regulates nox1 and nox4-based NADPH oxidase in human aortic smooth muscle cells. Arterioscler Thromb Vasc Biol. 2010 Jan;30(1):105-12.
REF 24 Mechanistic insights into activation and SOCS3-mediated inhibition of myeloproliferative neoplasm-associated JAK2 mutants from biochemical and structural analyses. Biochem J. 2014 Mar 1;458(2):395-405.
REF 25 Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1. Mol Cell Biol. 2002 May;22(10):3316-26.
REF 26 SHP-2 regulates SOCS-1-mediated Janus kinase-2 ubiquitination/degradation downstream of the prolactin receptor. J Biol Chem. 2003 Dec 26;278(52):52021-31.
REF 27 The TEL-Jak2 oncoprotein induces Socs1 expression and altered cytokine response in Ba/F3 cells. Oncogene. 2001 Feb 15;20(7):849-58.
REF 28 JAK2 and SHP2 reciprocally regulate tyrosine phosphorylation and stability of proapoptotic protein ASK1. J Biol Chem. 2009 May 15;284(20):13481-8.
REF 29 Negative regulation of FAK signaling by SOCS proteins. EMBO J. 2003 Oct 1;22(19):5036-46.
REF 30 Cloning and characterization of novel CIS family genes. Biochem Biophys Res Commun. 1997 Oct 20;239(2):439-46.
REF 31 The JAK-binding protein JAB inhibits Janus tyrosine kinase activity through binding in the activation loop. EMBO J. 1999 Mar 1;18(5):1309-20.
REF 32 A new protein containing an SH2 domain that inhibits JAK kinases. Nature. 1997 Jun 26;387(6636):921-4.
REF 33 CIS3/SOCS-3 suppresses erythropoietin (EPO) signaling by binding the EPO receptor and JAK2. J Biol Chem. 2000 Sep 22;275(38):29338-47.
REF 34 Suppressor of cytokine signaling-3 Provides a novel interface in the cross-talk between angiotensin II and insulin signaling systems. Endocrinology. 2005 Feb;146(2):579-88.
REF 35 Cytokine-inducible SH2 protein-3 (CIS3/SOCS3) inhibits Janus tyrosine kinase by binding through the N-terminal kinase inhibitory region as well as SH2 domain. Genes Cells. 1999 Jun;4(6):339-51.
REF 36 Role of the vav proto-oncogene product (Vav) in erythropoietin-mediated cell proliferation and phosphatidylinositol 3-kinase activity. J Biol Chem. 1997 May 30;272(22):14334-40.
REF 37 Tyrosine phosphorylation of p95Vav in myeloid cells is regulated by GM-CSF, IL-3 and steel factor and is constitutively increased by p210BCR/ABL. EMBO J. 1995 Jan 16;14(2):257-65.
REF 38 Induction of Jak/STAT signaling by activation of the type 1 TNF receptor. J Immunol. 1998 Mar 15;160(6):2742-50.
REF 39 Signal transducer and activator of transcription (STAT)-induced STAT inhibitor 1 (SSI-1)/suppressor of cytokine signaling 1 (SOCS1) inhibits insulin signal transduction pathway through modulating insulin receptor substrate 1 (IRS-1) phosphorylation. J Exp Med. 2001 Jan 15;193(2):263-9.
REF 40 Interaction of Janus kinases JAK-1 and JAK-2 with the insulin receptor and the insulin-like growth factor-1 receptor. Endocrinology. 1998 Mar;139(3):884-93.

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