Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T25265 Target Info
Target Name Enhancer of zeste homolog 2 (EZH2)
Synonyms Lysine Nmethyltransferase 6; Lysine N-methyltransferase 6; KMT6; Histonelysine Nmethyltransferase EZH2; Histone-lysine N-methyltransferase EZH2; EZH2; ENX1; ENX-1
Target Type Successful Target
Gene Name EZH2
Biochemical Class Methyltransferase
UniProt ID
EZH2_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 2 Acid anhydride hydrolases +
Interacting Protein Name Chromodomain-helicase-DNA-binding protein 4 (CHD4) Interacting Protein Interacting Protein Info
UniProt ID
CHD4_HUMAN 
Synonyms CHD.4; Mi.2b; Mi2.BETA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
3 Co-fractionation [3]
Interacting Target Name ATP-dependent RNA helicase DDX5 (DDX5) Clinical trial Target Interacting Protein Info
UniProt ID
DDX5_HUMAN 
Synonyms RNA helicase p68; Probable ATP-dependent RNA helicase DDX5; HLR1; HELR; G17P1; DEAD box protein 5
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-Western [5]
Acyltransferases [+] 1 Acyltransferases +
Interacting Target Name Transcription intermediary factor 1-beta (TRIM28) Literature-reported Target Interacting Protein Info
UniProt ID
TIF1B_HUMAN 
Synonyms Tripartite motif-containing protein 28; TIF1-beta; TIF1B; RNF96; RING-type E3 ubiquitin transferase TIF1-beta; RING finger protein 96; Nuclear corepressor KAP-1; KRIP-1; KRAB-interacting protein 1; KRAB-associated protein 1; KAP-1; KAP1; E3 SUMO-protein ligase TRIM28
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-MS [1]
3 Affinity Capture-Western [1]
4 Co-localization [1]
5 Reconstituted Complex [1]
Beta-transducin repeats [+] 2 Beta-transducin repeats +
Interacting Target Name Embryonic ectoderm development protein (EED) Clinical trial Target Interacting Protein Info
UniProt ID
EED_HUMAN 
Synonyms WD protein associating with integrin cytoplasmic tails 1; WAIT-1; hEED; Embryonic ectoderm development protein
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [7]
4 Affinity Capture-MS [1]
5 Affinity Capture-Western [8]
6 Affinity Capture-Western [9]
7 Affinity Capture-Western [10]
8 Affinity Capture-Western [11]
9 Affinity Capture-Western [12]
10 Affinity Capture-Western [5]
11 Affinity Capture-Western [13]
12 Affinity Capture-Western [14]
13 Affinity Capture-Western [15]
14 Affinity Capture-Western [16]
15 Affinity Capture-Western [17]
16 Affinity Capture-Western [18]
17 Affinity Capture-Western [19]
18 Affinity Capture-Western [20]
19 Affinity Capture-Western [21]
20 Affinity Capture-Western [22]
Interacting Protein Name Histone-binding protein RBBP4 (RBBP4) Interacting Protein Interacting Protein Info
UniProt ID
RBBP4_HUMAN 
Synonyms NURF55; RBAP48; lin.53
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [7]
4 Affinity Capture-MS [23]
5 Affinity Capture-Western [1]
6 Biochemical Activity [24]
7 Reconstituted Complex [24]
Carbon-nitrogen hydrolases [+] 3 Carbon-nitrogen hydrolases +
Interacting Target Name Histone deacetylase 1 (HDAC1) Successful Target Interacting Protein Info
UniProt ID
HDAC1_HUMAN 
Synonyms RPD3L1; HD1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [25]
2 Affinity Capture-Western [13]
3 Affinity Capture-Western [26]
4 Affinity Capture-Western [27]
5 Co-fractionation [3]
Interacting Target Name Histone deacetylase 2 (HDAC2) Clinical trial Target Interacting Protein Info
UniProt ID
HDAC2_HUMAN 
Synonyms HD2
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [26]
2 Affinity Capture-Western [13]
3 Co-fractionation [3]
Interacting Target Name NAD-dependent deacetylase sirtuin-1 (SIRT1) Clinical trial Target Interacting Protein Info
UniProt ID
SIR1_HUMAN 
Synonyms SIR2-like protein 1; SIR2L1; Regulatory protein SIR2 homolog 1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; hSIR2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [28]
2 Biochemical Activity [29]
Histones [+] 1 Histones +
Interacting Protein Name Histone H3.1 (H31) Interacting Protein Interacting Protein Info
UniProt ID
H31_HUMAN 
Synonyms H3/A; H3FA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [30]
2 Biochemical Activity [31]
3 Biochemical Activity [30]
Kinases [+] 1 Kinases +
Interacting Target Name Cyclin-dependent kinase 1 (CDK1) Clinical trial Target Interacting Protein Info
UniProt ID
CDK1_HUMAN 
Synonyms P34CDC2; P34 protein kinase; CDKN1; CDC28A; CDC2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [32]
2 Biochemical Activity [33]
One-carbon transferases [+] 1 One-carbon transferases +
Interacting Target Name DNA [cytosine-5]-methyltransferase 1 (DNMT1) Clinical trial Target Interacting Protein Info
UniProt ID
DNMT1_HUMAN 
Synonyms MCMT; M.HsaI; Dnmt1; DNMT; DNA MTase HsaI; DNA methyltransferase HsaI; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine5)methyltransferase 1; CXXC-type zinc finger protein 9; CXXCtype zinc finger protein 9; CXXC9; AIM
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [34]
3 Affinity Capture-Western [35]
4 Reconstituted Complex [34]
Transcription regulators [+] 1 Transcription regulators +
Interacting Protein Name Metal-response element-binding transcription factor 2 (MTF2) Interacting Protein Interacting Protein Info
UniProt ID
MTF2_HUMAN 
Synonyms M96; PCL2; TDRD19A; dJ976O13.2
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-MS [7]
3 Affinity Capture-MS [23]
4 Affinity Capture-Western [23]
Other proteins [+] 3 Other proteins +
Interacting Protein Name Polycomb protein SUZ12 (SUZ12) Interacting Protein Interacting Protein Info
UniProt ID
SUZ12_HUMAN 
Synonyms CHET9; JJAZ1
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [7]
4 Affinity Capture-MS [1]
5 Affinity Capture-MS [23]
6 Affinity Capture-Western [8]
7 Affinity Capture-Western [4]
8 Affinity Capture-Western [36]
9 Affinity Capture-Western [9]
10 Affinity Capture-Western [10]
11 Affinity Capture-Western [11]
12 Affinity Capture-Western [1]
13 Affinity Capture-Western [5]
14 Affinity Capture-Western [35]
15 Affinity Capture-Western [15]
16 Affinity Capture-Western [16]
17 Affinity Capture-Western [18]
18 Affinity Capture-Western [19]
19 Affinity Capture-Western [22]
20 Affinity Capture-Western [37]
Interacting Protein Name Protein Jumonji (JARID2) Interacting Protein Interacting Protein Info
UniProt ID
JARD2_HUMAN 
Synonyms JMJ
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [7]
4 Affinity Capture-MS [23]
5 Affinity Capture-Western [8]
6 Affinity Capture-Western [23]
Interacting Target Name Beta-catenin (CTNNB1) Successful Target Interacting Protein Info
UniProt ID
CTNB1_HUMAN 
Synonyms Wnt/beta-catenin signaling pathway; PRO2286; OK/SW-cl.35; CTNNB; Catenin beta-1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [38]
2 Affinity Capture-Western [39]
3 Reconstituted Complex [39]
References  Top
REF 1 TRIM28 interacts with EZH2 and SWI/SNF to activate genes that promote mammosphere formation. Oncogene. 2017 May 25;36(21):2991-3001.
REF 2 CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017 May 8;31(5):653-668.e7.
REF 3 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 4 The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127.
REF 5 RNA helicase DEAD box protein 5 regulates Polycomb repressive complex 2/Hox transcript antisense intergenic RNA function in hepatitis B virus infection and hepatocarcinogenesis. Hepatology. 2016 Oct;64(4):1033-48.
REF 6 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 7 A High-Density Map for Navigating the Human Polycomb Complexome. Cell Rep. 2016 Oct 4;17(2):583-595.
REF 8 JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in ES cells. Nature. 2010 Mar 11;464(7286):306-10.
REF 9 Fine-tuning the stimulation of MLL1 methyltransferase activity by a histone H3-based peptide mimetic. FASEB J. 2011 Mar;25(3):960-7.
REF 10 Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol Cell. 2008 Nov 21;32(4):503-18.
REF 11 EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol Cell. 2008 Nov 21;32(4):491-502.
REF 12 Interaction of mouse polycomb-group (Pc-G) proteins Enx1 and Enx2 with Eed: indication for separate Pc-G complexes. Mol Cell Biol. 1998 Jun;18(6):3572-9.
REF 13 Transcriptional repression mediated by the human polycomb-group protein EED involves histone deacetylation. Nat Genet. 1999 Dec;23(4):474-8.
REF 14 Point mutations in the WD40 domain of Eed block its interaction with Ezh2. Mol Cell Biol. 1998 Oct;18(10):5634-42.
REF 15 Polycomb group protein ezh2 controls actin polymerization and cell signaling. Cell. 2005 May 6;121(3):425-36.
REF 16 A model for transmission of the H3K27me3 epigenetic mark. Nat Cell Biol. 2008 Nov;10(11):1291-300.
REF 17 Association of BMI1 with polycomb bodies is dynamic and requires PRC2/EZH2 and the maintenance DNA methyltransferase DNMT1. Mol Cell Biol. 2005 Dec;25(24):11047-58.
REF 18 Ezh2 requires PHF1 to efficiently catalyze H3 lysine 27 trimethylation in vivo. Mol Cell Biol. 2008 Apr;28(8):2718-31.
REF 19 Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethylation. Blood. 2011 Feb 24;117(8):2451-9.
REF 20 The polycomb group protein EED interacts with YY1, and both proteins induce neural tissue in Xenopus embryos. Mol Cell Biol. 2001 Feb;21(4):1360-9.
REF 21 Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes. Mol Cell Biol. 1998 Jun;18(6):3586-95.
REF 22 BRCA1 is a negative modulator of the PRC2 complex. EMBO J. 2013 May 29;32(11):1584-97.
REF 23 Jarid2 and PRC2, partners in regulating gene expression. Genes Dev. 2010 Feb 15;24(4):368-80.
REF 24 Phosphorylation of the PRC2 component Ezh2 is cell cycle-regulated and up-regulates its binding to ncRNA. Genes Dev. 2010 Dec 1;24(23):2615-20.
REF 25 The Polycomb Ezh2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation. Genes Dev. 2004 Nov 1;18(21):2627-38.
REF 26 EZH2 supports nasopharyngeal carcinoma cell aggressiveness by forming a co-repressor complex with HDAC1/HDAC2 and Snail to inhibit E-cadherin. Oncogene. 2012 Feb 2;31(5):583-94.
REF 27 Deconstruction of the SS18-SSX fusion oncoprotein complex: insights into disease etiology and therapeutics. Cancer Cell. 2012 Mar 20;21(3):333-47.
REF 28 Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation. Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1859-64.
REF 29 PCAF-primed EZH2 acetylation regulates its stability and promotes lung adenocarcinoma progression. Nucleic Acids Res. 2015 Apr 20;43(7):3591-604.
REF 30 Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3. Science. 2005 Oct 14;310(5746):306-10.
REF 31 Substrate preferences of the EZH2 histone methyltransferase complex. J Biol Chem. 2006 Mar 31;281(13):8365-70.
REF 32 CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells. Nat Cell Biol. 2011 Jan;13(1):87-94.
REF 33 Cyclin-dependent kinases regulate epigenetic gene silencing through phosphorylation of EZH2. Nat Cell Biol. 2010 Nov;12(11):1108-14.
REF 34 The Polycomb group protein EZH2 directly controls DNA methylation. Nature. 2006 Feb 16;439(7078):871-4.
REF 35 Tumor suppressor menin represses paired box gene 2 expression via Wilms tumor suppressor protein-polycomb group complex. J Biol Chem. 2011 Apr 22;286(16):13937-44.
REF 36 Polycomb group protein-associated chromatin is reproduced in post-mitotic G1 phase and is required for S phase progression. J Biol Chem. 2008 Jul 4;283(27):18905-15.
REF 37 ZRANB1 Is an EZH2 Deubiquitinase and a Potential Therapeutic Target in Breast Cancer. Cell Rep. 2018 Apr 17;23(3):823-837.
REF 38 Targeted overexpression of EZH2 in the mammary gland disrupts ductal morphogenesis and causes epithelial hyperplasia. Am J Pathol. 2009 Sep;175(3):1246-54.
REF 39 Integration of estrogen and Wnt signaling circuits by the polycomb group protein EZH2 in breast cancer cells. Mol Cell Biol. 2007 Jul;27(14):5105-19.

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