Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T70532 Target Info
Target Name NCK adaptor protein 1 (NCK1)
Synonyms SH2/SH3 adaptor protein NCKalpha; SH2/SH3 adaptor protein NCK-alpha; Nck1; Nck-1; NCK; Cytoplasmic protein NCK1
Target Type Clinical trial Target
Gene Name NCK1
UniProt ID
NCK1_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 1 Acyltransferases +
Interacting Target Name Signal transduction protein CBL (CBL) Literature-reported Target Interacting Protein Info
UniProt ID
CBL_HUMAN 
Synonyms RNF55; RING-type E3 ubiquitin transferase CBL; RING finger protein 55; Proto-oncogene c-CBL; E3 ubiquitin-protein ligase CBL; C-Cbl; CBL2; Casitas B-lineage lymphoma proto-oncogene
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Reconstituted Complex [4]
6 Reconstituted Complex [5]
7 Reconstituted Complex [3]
Kinases [+] 6 Kinases +
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [6]
2 Affinity Capture-Western [7]
3 Protein-Fragment Complementation Assay [8]
4 Protein-peptide [9]
5 Reconstituted Complex [10]
6 Yeast two hybrid [11]
Interacting Target Name MEK kinase kinase 1 (MAP4K1) Clinical trial Target Interacting Protein Info
UniProt ID
M4K1_HUMAN 
Synonyms Mitogen-activated protein kinase kinase kinase kinase 1; MEKKK 1; MAPK/ERK kinase kinase kinase 1; HPK1; Hematopoietic progenitor kinase
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Protein-peptide [13]
3 Reconstituted Complex [14]
4 Yeast two hybrid [15]
Interacting Target Name PAK-1 protein kinase (PAK1) Literature-reported Target Interacting Protein Info
UniProt ID
PAK1_HUMAN 
Synonyms Serine/threonine-protein kinase PAK 1; PAK-1; p65-PAK; p21-activated kinase 1; Alpha-PAK
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [16]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [7]
4 Reconstituted Complex [17]
Interacting Target Name Tyrosine-protein kinase ABL1 (ABL) Successful Target Interacting Protein Info
UniProt ID
ABL1_HUMAN 
Synonyms Proto-oncogene tyrosine-protein kinase ABL1; Proto-oncogene c-Abl; p150; JTK7; C-ABL; Abl; Abelson tyrosine-protein kinase 1; Abelson murine leukemia viral oncogene homolog 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [18]
2 Reconstituted Complex [19]
3 Yeast two hybrid [19]
Interacting Target Name Focal adhesion kinase 1 (FAK) Clinical trial Target Interacting Protein Info
UniProt ID
FAK1_HUMAN 
Synonyms Protein-tyrosine kinase 2; Protein phosphatase 1 regulatory subunit 71; PPP1R71; pp125FAK; p125FAK; FRNK; Focal adhesion kinase-related nonkinase; FAK1; FADK 1; FADK
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [20]
2 Reconstituted Complex [21]
3 Reconstituted Complex [20]
Interacting Target Name Protein kinase N2 (PKN2) Literature-reported Target Interacting Protein Info
UniProt ID
PKN2_HUMAN 
Synonyms Serine/threonine-protein kinase N2; Protein-kinase C-related kinase 2; Protein kinase C-like 2; PRKCL2; PRK2; PKN gamma
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Reconstituted Complex [22]
Other proteins [+] 7 Other proteins +
Interacting Protein Name Son of sevenless homolog 1 (SOS1) Interacting Protein Interacting Protein Info
UniProt ID
SOS1_HUMAN 
Synonyms GF1; GGF1; GINGF; HGF; NS4
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [23]
2 Affinity Capture-Western [7]
3 Affinity Capture-Western [24]
4 Reconstituted Complex [24]
5 Reconstituted Complex [25]
Interacting Target Name GAP-associated tyrosine phosphoprotein p62 (KHDRBS1) Literature-reported Target Interacting Protein Info
UniProt ID
KHDR1_HUMAN 
Synonyms Src-associated in mitosis 68 kDa protein; Srcassociated in mitosis 68 kDa protein; Sam68; p68; p21 Ras GTPase-activating protein-associated p62; p21 Ras GTPaseactivating proteinassociated p62; KH domain-containing, RNA-binding, signal transduction-associated protein 1; KH domaincontaining, RNAbinding, signal transductionassociated protein 1; GAPassociated tyrosine phosphoprotein p62
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [26]
2 Affinity Capture-Western [27]
3 Far Western [26]
4 Reconstituted Complex [10]
Interacting Protein Name Neural Wiskott-Aldrich syndrome protein (WASL) Interacting Protein Interacting Protein Info
UniProt ID
WASL_HUMAN 
Synonyms N.WASP; NWASP
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [28]
2 Reconstituted Complex [29]
3 Yeast two hybrid [30]
Interacting Target Name Wiskott-Aldrich syndrome protein (WAS) Clinical trial Target Interacting Protein Info
UniProt ID
WASP_HUMAN 
Synonyms WASp; IMD2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [31]
2 Reconstituted Complex [32]
Interacting Target Name Apoptosis antigen ligand (CD178) Literature-reported Target Interacting Protein Info
UniProt ID
TNFL6_HUMAN 
Synonyms Tumor necrosis factor ligand superfamily member 6; TNFSF6; FasL; Fas ligand; FAS antigen ligand; CD95-L; CD95L; CD95 ligand; CD178 antigen; CD178; APTL; APT1LG1; Apoptosis antigen ligand
Evidence Score (E-score) 2 Experimental Evidences +
1 Reconstituted Complex [33]
2 Yeast two hybrid [30]
Interacting Target Name GTPase activating protein (RASA1) Literature-reported Target Interacting Protein Info
UniProt ID
RASA1_HUMAN 
Synonyms RasGAP; RASA; Ras p21 protein activator; Ras GTPase-activating protein 1; p120GAP; GTPase-activating protein; GAP
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [34]
2 Far Western [35]
Interacting Protein Name Lymphocyte cytosolic protein 2 (LCP2) Interacting Protein Interacting Protein Info
UniProt ID
LCP2_HUMAN 
Synonyms SLP.76; SLP76
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [36]
2 Reconstituted Complex [37]
References  Top
REF 1 Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor. Oncogene. 2001 Jul 5;20(30):4058-69.
REF 2 Characterization of Cbl-Nck and Nck-Pak1 interactions in myeloid FcgammaRII signaling. Exp Cell Res. 1998 Dec 15;245(2):330-42.
REF 3 Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. Nat Commun. 2013;4:2863.
REF 4 Abl family kinases and Cbl cooperate with the Nck adaptor to modulate Xenopus development. J Biol Chem. 2000 Nov 17;275(46):36472-8.
REF 5 The kinase-deficient Src acts as a suppressor of the Abl kinase for Cbl phosphorylation. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6439-44.
REF 6 The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors. Mol Cell Biol. 1992 Dec;12(12):5824-33.
REF 7 Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck. J Biol Chem. 1999 Feb 26;274(9):5542-9.
REF 8 The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nat Methods. 2014 May;11(5):585-92.
REF 9 A quantitative protein interaction network for the ErbB receptors using protein microarrays. Nature. 2006 Jan 12;439(7073):168-74.
REF 10 Induced direct binding of the adapter protein Nck to the GTPase-activating protein-associated protein p62 by epidermal growth factor. Oncogene. 1997 Oct 9;15(15):1823-32.
REF 11 In silico prediction of physical protein interactions and characterization of interactome orphans. Nat Methods. 2015 Jan;12(1):79-84.
REF 12 Involvement of hematopoietic progenitor kinase 1 in T cell receptor signaling. J Biol Chem. 2001 Jun 1;276(22):18908-14.
REF 13 SRC Homology 2 Domain Binding Sites in Insulin, IGF-1 and FGF receptor mediated signaling networks reveal an extensive potential interactome. Cell Commun Signal. 2012 Sep 14;10(1):27.
REF 14 Interaction of hematopoietic progenitor kinase 1 with adapter proteins Crk and CrkL leads to synergistic activation of c-Jun N-terminal kinase. Mol Cell Biol. 1999 Feb;19(2):1359-68.
REF 15 SH2/SH3 adaptor proteins can link tyrosine kinases to a Ste20-related protein kinase, HPK1. J Biol Chem. 1997 Oct 31;272(44):27804-11.
REF 16 A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT. EMBO J. 2001 Feb 1;20(3):457-65.
REF 17 Interaction of the Nck adapter protein with p21-activated kinase (PAK1). J Biol Chem. 1996 Oct 18;271(42):25746-9.
REF 18 Requirement of multiple SH3 domains of Nck for ligand binding. Cell Signal. 1999 Apr;11(4):253-62.
REF 19 Abl protein-tyrosine kinase selects the Crk adapter as a substrate using SH3-binding sites. Genes Dev. 1994 Apr 1;8(7):783-95.
REF 20 Nck-2 interacts with focal adhesion kinase and modulates cell motility. Int J Biochem Cell Biol. 2002 Jul;34(7):791-805.
REF 21 Structure and function of Cas-L, a 105-kD Crk-associated substrate-related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes. J Exp Med. 1996 Oct 1;184(4):1365-75.
REF 22 Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling. J Biol Chem. 1996 Nov 15;271(46):28772-6.
REF 23 Characterization of interactions of Nck with Sos and dynamin. Cell Signal. 1999 Jan;11(1):25-9.
REF 24 Binding of NCK to SOS and activation of ras-dependent gene expression. Mol Cell Biol. 1995 Mar;15(3):1169-74.
REF 25 Interactions between Src homology (SH) 2/SH3 adapter proteins and the guanylnucleotide exchange factor SOS are differentially regulated by insulin and epidermal growth factor. J Biol Chem. 1996 Oct 11;271(41):25533-8.
REF 26 The Nck SH2/SH3 adaptor protein is present in the nucleus and associates with the nuclear protein SAM68. Oncogene. 1997 Jan 16;14(2):223-31.
REF 27 Comprehensive analysis of interactions between the Src-associated protein in mitosis of 68 kDa and the human Src-homology 3 proteome. PLoS One. 2012;7(6):e38540.
REF 28 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 29 Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway. J Biol Chem. 2001 Jul 13;276(28):26448-52.
REF 30 Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing. Cell. 2016 Feb 11;164(4):805-17.
REF 31 Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton. J Cell Biol. 2000 Apr 3;149(1):181-94.
REF 32 Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains. Mol Cell Biol. 1995 Oct;15(10):5725-31.
REF 33 Multiple interactions of the cytosolic polyproline region of the CD95 ligand: hints for the reverse signal transduction capacity of a death factor. FEBS Lett. 2001 Dec 7;509(2):255-62.
REF 34 Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J. 1997 Jul 1;16(13):3877-88.
REF 35 Adaptor protein Nck1 interacts with p120 Ras GTPase-activating protein and regulates its activity. Cell Signal. 2011 Oct;23(10):1651-8.
REF 36 Once phosphorylated, tyrosines in carboxyl terminus of protein-tyrosine kinase Syk interact with signaling proteins, including TULA-2, a negative regulator of mast cell degranulation. J Biol Chem. 2012 Mar 9;287(11):8194-204.
REF 37 Association of Nck with tyrosine-phosphorylated SLP-76 in activated T lymphocytes. Eur J Immunol. 1999 Apr;29(4):1068-75.

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