Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T83982 Target Info
Target Name Ubiquitin isopeptidase Y (USP8)
Synonyms hUBPy; Ubiquitin-specific-processing protease 8; Ubiquitin thioesterase 8; Ubiquitin carboxyl-terminal hydrolase 8; UBPY; KIAA0055; Deubiquitinating enzyme 8
Target Type Preclinical Target
Gene Name USP8
Biochemical Class Peptidase
UniProt ID
UBP8_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 1 Acyltransferases +
Interacting Protein Name E3 ubiquitin-protein ligase NRDP1 (RNF41) Interacting Protein Interacting Protein Info
UniProt ID
RNF41_HUMAN 
Synonyms FLRF; NRDP1
Evidence Score (E-score) 3 Experimental Evidences +
1 Co-crystal Structure [1]
2 Fluorescence Resonance Energy Transfer [2]
3 Reconstituted Complex [1]
Adapter proteins [+] 3 Adapter proteins +
Interacting Protein Name Signal transducing adapter molecule 1 (STAM) Interacting Protein Interacting Protein Info
UniProt ID
STAM1_HUMAN 
Synonyms STAM.1; STAM1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Protein-Fragment Complementation Assay [4]
3 Reconstituted Complex [5]
4 Reconstituted Complex [6]
Interacting Protein Name Signal transducing adapter molecule 2 (STAM2) Interacting Protein Interacting Protein Info
UniProt ID
STAM2_HUMAN 
Synonyms Hbp; STAM2A; STAM2B
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [5]
2 Protein-Fragment Complementation Assay [4]
3 Reconstituted Complex [5]
4 Reconstituted Complex [6]
Interacting Protein Name GRB2-related adapter protein 2 (GRAP2) Interacting Protein Interacting Protein Info
UniProt ID
GRAP2_HUMAN 
Synonyms GADS; GRAP.2; GRB2L; GRBLG; GRID; GRPL; GrbX; Grf40; Mona; P38
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Protein-peptide [8]
Kinases [+] 1 Kinases +
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Affinity Capture-Western [10]
3 Biochemical Activity [11]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Target Name Polyubiquitin-C (UBC) Patented-recorded Target Interacting Protein Info
UniProt ID
UBC_HUMAN 
Synonyms HMG20; ubiquitin C
Evidence Score (E-score) 7 Experimental Evidences +
1 Biochemical Activity [12]
2 Biochemical Activity [13]
3 Biochemical Activity [14]
4 Biochemical Activity [15]
5 Biochemical Activity [11]
6 Co-crystal Structure [16]
7 Protein-peptide [16]
Other proteins [+] 1 Other proteins +
Interacting Protein Name Charged multivesicular body protein 1b (CHMP1B) Interacting Protein Interacting Protein Info
UniProt ID
CHM1B_HUMAN 
Synonyms C10orf2; C18.ORF2; C18orf2; CHMP1.5; Vps46.2; Vps46B; hVps46.2
Evidence Score (E-score) 3 Experimental Evidences +
1 Reconstituted Complex [15]
2 Reconstituted Complex [17]
3 Yeast two hybrid [15]
References  Top
REF 1 Amino-terminal dimerization, NRDP1-rhodanese interaction, and inhibited catalytic domain conformation of the ubiquitin-specific protease 8 (USP8). J Biol Chem. 2006 Dec 8;281(49):38061-70.
REF 2 Reciprocal cross-regulation between RNF41 and USP8 controls cytokine receptor sorting and processing. J Cell Sci. 2013 Aug 15;126(Pt 16):3770-81.
REF 3 The ubiquitin isopeptidase UBPY regulates endosomal ubiquitin dynamics and is essential for receptor down-regulation. J Biol Chem. 2006 May 5;281(18):12618-24.
REF 4 Regulation of epidermal growth factor receptor ubiquitination and trafficking by the USP8TAM complex. J Biol Chem. 2010 Nov 5;285(45):34909-21.
REF 5 A deubiquitinating enzyme UBPY interacts with the Src homology 3 domain of Hrs-binding protein via a novel binding motif PX(V/I)(D/N)RXXKP. J Biol Chem. 2000 Dec 1;275(48):37481-7.
REF 6 Ubiquitin-specific protease 8 deubiquitinates Sec31A and decreases large COPII carriers and collagen IV secretion. Biochem Biophys Res Commun. 2018 May 15;499(3):635-641.
REF 7 The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells. Nat Immunol. 2015 Sep;16(9):950-60.
REF 8 A high-affinity Arg-X-X-Lys SH3 binding motif confers specificity for the interaction between Gads and SLP-76 in T cell signaling. Curr Biol. 2002 Aug 6;12(15):1336-41.
REF 9 Inhibition of Ubiquitin-specific Peptidase 8 Suppresses Growth of Gefitinib-resistant Non-small Cell Lung Cancer Cells by Inducing Apoptosis. J Cancer Prev. 2015 Mar;20(1):57-63.
REF 10 Cbl and Itch binding sites in ERBB4 CYT-1 and CYT-2 mediate K48- and K63-polyubiquitination, respectively. Cell Signal. 2013 Feb;25(2):470-8.
REF 11 Mutations in the deubiquitinase gene USP8 cause Cushing's disease. Nat Genet. 2015 Jan;47(1):31-8.
REF 12 The differential modulation of USP activity by internal regulatory domains, interactors and eight ubiquitin chain types. Chem Biol. 2011 Dec 23;18(12):1550-61.
REF 13 Ubiquitin Ser65 phosphorylation affects ubiquitin structure, chain assembly and hydrolysis. EMBO J. 2015 Feb 3;34(3):307-25.
REF 14 Divergence in Ubiquitin Interaction and Catalysis among the Ubiquitin-Specific Protease Family Deubiquitinating Enzymes. Biochemistry. 2016 Aug 23;55(33):4708-19.
REF 15 The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degrada... J Biol Chem. 2007 Oct 19;282(42):30929-37.
REF 16 A strategy for modulation of enzymes in the ubiquitin system. Science. 2013 Feb 1;339(6119):590-5.
REF 17 Ab initio protein modelling reveals novel human MIT domains. FEBS Lett. 2009 Mar 4;583(5):872-8.

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