| Target Validation Information | |||||
|---|---|---|---|---|---|
| Target ID | T23471 | ||||
| Target Name | Myeloperoxidase | ||||
| Target Type | Clinical Trial |
||||
| Action against Disease Model | 4-aminobenzoic acid hydrazide | Pretreatment of MPO-high leukemia cells with a MPO-specific inhibitor, 4-aminobenzoic acid hydrazide, or a MPO-specific small interfering RNA (siRNA) abrogated the PTL-induced ROS generation and apoptosis, indicating that MPO plays a crucial role in PTL-induced apoptosis in leukemia cells. | [531097] | Drug Info | |
| The Effect of Target Knockout, Knockdown or Genetic Variations | Here, we determined possible mechanisms protecting the host using MPO(-/-) mice challenged with live gram-negative bacteri uM Escherichia coli. We observed that MPO(-/-) mice unexpectedly had improved survival compared with wild-type (WT) mice within 5-12 h after intraperitoneal E. coli challenge. Lungs of MPO(-/-) mice also demonstrated lower bacterial colonization and markedly attenuated increases in microvascular permeability and edema formation after E. coli challenge compared with WT. However, PMN sequestration in lungs of both groups was similar. Basal inducible nitric oxide synthase (iNOS) expression was significantly elevated in lungs and PMNs of MPO(-/-) mice, and NO production was increased two- to sixfold compared with WT. Nitrotyrosine levels doubled in lungs of WT mice within 1 h after E. coli challenge but did not change in MPO(-/-) mice. Inhibition of iNOS in MPO(-/-) mice significantly increased lung edema and reduced their survival after E. coli challenge, but iNOS inhibitor had the opposite effect in WT mice. Thus augmented iNOS expression and NO production in MPO(-/-) mice compensate for the lack of HOCl-mediated bacterial killing, and the absence of MPO-derived oxidants mitigates E. coli sepsis-induced lung inflammation and injury. | [531097] | |||
| References | |||||
| Ref 531097 | J Pharmacol Exp Ther. 2010 Nov;335(2):389-400. Epub 2010 Aug 10.Myeloperoxidase expression as a potential determinant of parthenolide-induced apoptosis in leukemia bulk and leukemia stem cells. | ||||
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