Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T23673 Target Info
Target Name Proto-oncogene c-RAF (c-RAF)
Synonyms cRaf; Raf-1; RAF proto-oncogene serine/threonine-protein kinase; RAF
Target Type Clinical trial Target
Gene Name RAF1
Biochemical Class Kinase
UniProt ID
RAF1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 3 Acid anhydride hydrolases +
Interacting Target Name GTPase HRas (HRAS) Literature-reported Target Interacting Protein Info
UniProt ID
RASH_HUMAN 
Synonyms Transforming protein p21; p21ras; H-Ras-1; Ha-Ras; HaRas; GTPase HRas, Nterminally processed; GTPase HRas; c-H-ras; cHras
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Fluorescence Resonance Energy Transfer [2]
3 Reconstituted Complex [3]
4 Yeast two hybrid [4]
Interacting Target Name GTPase KRas (KRAS) Clinical trial Target Interacting Protein Info
UniProt ID
RASK_HUMAN 
Synonyms K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [1]
3 Reconstituted Complex [6]
Interacting Protein Name Ras-related protein R-Ras (RRAS) Interacting Protein Interacting Protein Info
UniProt ID
RRAS_HUMAN 
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Yeast two hybrid [8]
Acyltransferases [+] 1 Acyltransferases +
Interacting Target Name X-linked inhibitor of apoptosis protein (XIAP) Clinical trial Target Interacting Protein Info
UniProt ID
XIAP_HUMAN 
Synonyms X-linked IAP; Xlinked IAP; RING-type E3 ubiquitin transferase XIAP; Inhibitor of apoptosis protein 3; ILP; IAP-like protein; IAPlike protein; IAP-3; IAP3; hILP; hIAP-3; hIAP3; E3 ubiquitin-protein ligase XIAP; E3 ubiquitinprotein ligase XIAP; BIRC4; Baculoviral IAP repeat-containing protein 4; Baculoviral IAP repeatcontaining protein 4; API3
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Biochemical Activity [10]
3 Yeast two hybrid [10]
Adapter proteins [+] 1 Adapter proteins +
Interacting Protein Name Growth factor receptor-bound protein 10 (GRB10) Interacting Protein Interacting Protein Info
UniProt ID
GRB10_HUMAN 
Synonyms GRB.IR; Grb.10; IRBP; MEG1; RSS
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [11]
2 Reconstituted Complex [12]
3 Yeast two hybrid [11]
Auxiliary transporters [+] 2 Auxiliary transporters +
Interacting Protein Name 14-3-3 protein zeta/delta (YWHAZ) Interacting Protein Interacting Protein Info
UniProt ID
1433Z_HUMAN 
Synonyms 14.3.3.zeta; HEL.S.3; HEL4; KCIP.1; YWHAD
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [13]
3 Fluorescence Resonance Energy Transfer [2]
4 Reconstituted Complex [13]
Interacting Target Name Apoptosis regulator Bcl-2 (BCL-2) Successful Target Interacting Protein Info
UniProt ID
BCL2_HUMAN 
Synonyms Bcl-2; Apoptosis regulator Bcl-2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [14]
2 Reconstituted Complex [15]
Cell division cycle proteins [+] 1 Cell division cycle proteins +
Interacting Protein Name Retinoblastoma-associated protein (RB1) Interacting Protein Interacting Protein Info
UniProt ID
RB_HUMAN 
Synonyms OSRC; PPP1R130; RB; p105.Rb; pRb; pp110
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [16]
2 Biochemical Activity [17]
3 Reconstituted Complex [17]
4 Yeast two hybrid [16]
Heat shock proteins [+] 3 Heat shock proteins +
Interacting Target Name Heat shock protein 90 alpha (HSP90A) Successful Target Interacting Protein Info
UniProt ID
HS90A_HUMAN 
Synonyms Renal carcinoma antigen NY-REN-38; LPS-associated protein 2; Lipopolysaccharide-associated protein 2; LAP-2; HSPCA; HSPC1; HSP90A; HSP86; HSP 86; Heat shock protein HSP 90-alpha; Heat shock 86 kDa
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [19]
Interacting Target Name Lipopolysaccharide-associated protein 1 (HSPA8) Literature-reported Target Interacting Protein Info
UniProt ID
HSP7C_HUMAN 
Synonyms LPS-associated protein 1; LAP-1; HSPA10; HSP73; HSC70; Heat shock protein 73; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [20]
2 Affinity Capture-Western [21]
Interacting Protein Name Heat shock 70 kDa protein 4 (HSPA4) Interacting Protein Interacting Protein Info
UniProt ID
HSP74_HUMAN 
Synonyms APG.2; HEL.S.5a; HS24/P52; HSPH2; RY; hsp70; hsp70RY
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [20]
2 Affinity Capture-Western [20]
Kinases [+] 8 Kinases +
Interacting Target Name ERK activator kinase 1 (MEK1) Clinical trial Target Interacting Protein Info
UniProt ID
MP2K1_HUMAN 
Synonyms PRKMK1; MKK1; Mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERKkinase 1; MAPK/ERK kinase 1; MAP kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Biochemical Activity [23]
3 Reconstituted Complex [24]
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 2 Experimental Evidences +
1 Protein-Fragment Complementation Assay [25]
2 Yeast two hybrid [26]
Interacting Target Name Serine/threonine-protein kinase B-raf (BRAF) Successful Target Interacting Protein Info
UniProt ID
BRAF_HUMAN 
Synonyms V-Raf murine sarcoma viral oncogene homolog B1; Serine/threonine-protein kinase B-raf; RAFB1; Proto-oncogene B-Raf; P94; BRAF1; BRAF(V599E); BRAF serine/threonine kinase; B-raf protein; B-Raf
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [27]
2 Affinity Capture-Western [28]
Interacting Target Name RAC-alpha serine/threonine-protein kinase (AKT1) Successful Target Interacting Protein Info
UniProt ID
AKT1_HUMAN 
Synonyms RAC-PK-alpha; RAC; Proto-oncogene c-Akt; Protein kinase B alpha; Protein kinase B; PKB alpha; PKB
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [29]
2 Fluorescence Resonance Energy Transfer [2]
Interacting Target Name Extracellular signal-regulated kinase 2 (ERK2) Clinical trial Target Interacting Protein Info
UniProt ID
MK01_HUMAN 
Synonyms PRKM2; PRKM1; P42-MAPK; P42 Mitogen-activated protein kinase; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; MAPK 2; MAPK 1; MAP kinase isoform p42; MAP kinase 2; MAP kinase 1; ERT1; ERK-2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [30]
2 Biochemical Activity [20]
Interacting Target Name ERK activator kinase 2 (MEK2) Clinical trial Target Interacting Protein Info
UniProt ID
MP2K2_HUMAN 
Synonyms PRKMK2; MKK2; MEK 2; MAPKK 2; MAPK/ERK kinase 2; MAP kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; Dual specificity mitogenactivated protein kinase kinase 2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [30]
2 Yeast two hybrid [31]
Interacting Target Name Apoptosis signal-regulating kinase 1 (MAP3K5) Clinical trial Target Interacting Protein Info
UniProt ID
M3K5_HUMAN 
Synonyms Mitogen-activated protein kinase kinase kinase 5; MEKK5; MEKK 5; MEK kinase 5; MAPKKK5; MAPK/ERK kinase kinase 5; ASK-1; ASK1
Evidence Score (E-score) 2 Experimental Evidences +
1 Fluorescence Resonance Energy Transfer [2]
2 Reconstituted Complex [32]
Interacting Protein Name Serine/threonine-protein kinase 3 (STK3) Interacting Protein Interacting Protein Info
UniProt ID
STK3_HUMAN 
Synonyms KRS1; MST2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [23]
Peptidases [+] 1 Peptidases +
Interacting Target Name CASP8-FADD-like regulator (CFLAR) Literature-reported Target Interacting Protein Info
UniProt ID
CFLAR_HUMAN 
Synonyms Usurpin; MRIT; MACH-related inducer of toxicity; MACHrelated inducer of toxicity; Inhibitor of FLICE; I-FLICE; IFLICE; FLAME-1; FLAME1; FADD-like antiapoptotic molecule 1; FADDlike antiapoptotic molecule 1; CLARP; c-FLIP; cFLIP; Cellular FLICE-like inhibitory protein; Cellular FLICElike inhibitory protein; Casper; Caspase-like apoptosis regulatory protein; Caspaselike apoptosis regulatory protein; Caspase-eight-related protein; Caspaseeightrelated protein; Caspase homolog; CASP8AP1; CASP8 and FADDlike apoptosis regulator subunit p12; CASP8 and FADD-like apoptosis regulator; CASP8 and FADDlike apoptosis regulator; CASH
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [33]
2 Yeast two hybrid [34]
Proteins 14-3-3 [+] 6 Proteins 14-3-3 +
Interacting Protein Name 14-3-3 protein beta/alpha (YWHAB) Interacting Protein Interacting Protein Info
UniProt ID
1433B_HUMAN 
Synonyms GW128; HEL.S.1; HS1; KCIP.1; YWHAA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [35]
3 Yeast two hybrid [36]
Interacting Protein Name 14-3-3 protein epsilon (YWHAE) Interacting Protein Interacting Protein Info
UniProt ID
1433E_HUMAN 
Synonyms 14.3.3E; HEL2; KCIP.1; MDCR; MDS
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [37]
3 Yeast two hybrid [38]
Interacting Protein Name 14-3-3 protein sigma (SFN) Interacting Protein Interacting Protein Info
UniProt ID
1433S_HUMAN 
Synonyms YWHAS
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [39]
3 Fluorescence Resonance Energy Transfer [40]
Interacting Target Name AS1 14-3-3 protein eta (YWHAH) Literature-reported Target Interacting Protein Info
UniProt ID
1433F_HUMAN 
Synonyms YWHA1; Protein AS1; 14-3-3 protein eta
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [41]
2 Affinity Capture-Western [35]
Interacting Protein Name 14-3-3 protein gamma (YWHAG) Interacting Protein Interacting Protein Info
UniProt ID
1433G_HUMAN 
Synonyms 14.3.3GAMMA; PPP1R170
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [35]
Interacting Protein Name 14-3-3 protein theta (YWHAQ) Interacting Protein Interacting Protein Info
UniProt ID
1433T_HUMAN 
Synonyms 14.3.3; 1C5; HS1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [35]
Transcription regulators [+] 1 Transcription regulators +
Interacting Target Name Proto-oncogene c-Myc (MYC) Literature-reported Target Interacting Protein Info
UniProt ID
MYC_HUMAN 
Synonyms Transcription factor p64; Proto-oncogene c-Myc; Myc proto-oncogene protein; C-myc oncogene; C-myc; Class E basic helix-loop-helix protein 39; bHLHe39
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [42]
2 Reconstituted Complex [43]
Other proteins [+] 4 Other proteins +
Interacting Target Name Phosphatidylethanolamine-binding protein 1 (PEBP1) Literature-reported Target Interacting Protein Info
UniProt ID
PEBP1_HUMAN 
Synonyms RKIP; Raf kinase inhibitor protein; Prostatic-binding protein; PEBP-1; PEBP; PBP; Neuropolypeptide h3; Hippocampal cholinergic neurostimulating peptide; HCNPpp; HCNP
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [44]
2 Reconstituted Complex [24]
Interacting Target Name Prohibitin (PHB) Literature-reported Target Interacting Protein Info
UniProt ID
PHB_HUMAN 
Synonyms PHB1; HEL-215; HEL-S-54e
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [45]
2 Reconstituted Complex [45]
Interacting Protein Name Ran-binding protein 9 (RANBP9) Interacting Protein Interacting Protein Info
UniProt ID
RANB9_HUMAN 
Synonyms BPM.L; BPM90; RANBPM; RanBP7
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [46]
2 Yeast two hybrid [31]
Interacting Protein Name TSC22 domain family protein 3 (TSC22D3) Interacting Protein Interacting Protein Info
UniProt ID
T22D3_HUMAN 
Synonyms DIP; DSIPI; GILZ; TSC.22R; hDIP
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [47]
2 Reconstituted Complex [48]
References  Top
REF 1 Stimulation of Ras guanine nucleotide exchange activity of Ras-GRF1/CDC25(Mm) upon tyrosine phosphorylation by the Cdc42-regulated kinase ACK1. J Biol Chem. 2000 Sep 22;275(38):29788-93.
REF 2 OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene. 2017 Oct 19;36(42):5852-5860.
REF 3 Identification and characterization of rain, a novel Ras-interacting protein with a unique subcellular localization. J Biol Chem. 2004 May 21;279(21):22353-61.
REF 4 SIAH-1 interacts with CtIP and promotes its degradation by the proteasome pathway. Oncogene. 2003 Dec 4;22(55):8845-51.
REF 5 Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy. Sci Signal. 2015 Apr 7;8(371):rs3.
REF 6 Hdm2 is regulated by K-Ras and mediates p53-independent functions in pancreatic cancer cells. Oncogene. 2009 Feb 5;28(5):709-20.
REF 7 Semaphorin 4D signaling requires the recruitment of phospholipase C gamma into the plexin-B1 receptor complex. Mol Cell Biol. 2009 Dec;29(23):6321-34.
REF 8 Isoform-specific localization of A-RAF in mitochondria. Mol Cell Biol. 2000 Jul;20(13):4870-8.
REF 9 X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility. Nat Cell Biol. 2008 Dec;10(12):1447-55.
REF 10 Interaction and stabilization of X-linked inhibitor of apoptosis by Raf-1 protein kinase. Int J Oncol. 2006 Oct;29(4):861-7.
REF 11 Localization of endogenous Grb10 to the mitochondria and its interaction with the mitochondrial-associated Raf-1 pool. J Biol Chem. 1999 Dec 10;274(50):35719-24.
REF 12 Interaction of the Grb10 adapter protein with the Raf1 and MEK1 kinases. J Biol Chem. 1998 Apr 24;273(17):10475-84.
REF 13 Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in intact cells. FEBS Lett. 1995 Jul 17;368(2):321-5.
REF 14 p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association. J Biol Chem. 2005 Jul 1;280(26):24698-705.
REF 15 Bcl-2 targets the protein kinase Raf-1 to mitochondria. Cell. 1996 Nov 15;87(4):629-38.
REF 16 Raf-1 physically interacts with Rb and regulates its function: a link between mitogenic signaling and cell cycle regulation. Mol Cell Biol. 1998 Dec;18(12):7487-98.
REF 17 Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis. Mol Cell Biol. 2004 Nov;24(21):9527-41.
REF 18 Role of the kinase MST2 in suppression of apoptosis by the proto-oncogene product Raf-1. Science. 2004 Dec 24;306(5705):2267-70.
REF 19 Raf exists in a native heterocomplex with hsp90 and p50 that can be reconstituted in a cell-free system. J Biol Chem. 1993 Oct 15;268(29):21711-6.
REF 20 Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding. Mol Biol Cell. 2005 Oct;16(10):4733-44.
REF 21 The enhancement of antiproliferative and proapoptotic activity of HDAC inhibitors by curcumin is mediated by Hsp90 inhibition. Cell Mol Life Sci. 2010 Mar;67(6):995-1004.
REF 22 G protein-coupled receptor-mediated mitogen-activated protein kinase activation through cooperation of Galpha(q) and Galpha(i) signals. Mol Cell Biol. 2000 Sep;20(18):6837-48.
REF 23 Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. J Biol Chem. 2010 May 14;285(20):15076-87.
REF 24 Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein. Mol Cell Biol. 2000 May;20(9):3079-85.
REF 25 The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nat Methods. 2014 May;11(5):585-92.
REF 26 In silico prediction of physical protein interactions and characterization of interactome orphans. Nat Methods. 2015 Jan;12(1):79-84.
REF 27 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 28 Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8.
REF 29 Phosphorylation and regulation of Raf by Akt (protein kinase B). Science. 1999 Nov 26;286(5445):1741-4.
REF 30 Akt regulates vitamin D3-induced leukemia cell functional differentiation via Raf/MEK/ERK MAPK signaling. Eur J Cell Biol. 2009 Feb;88(2):103-15.
REF 31 Ran binding protein 9 interacts with Raf kinase but does not contribute to downstream ERK1/2 activation in skeletal myoblasts. Biochem Biophys Res Commun. 2006 Feb 10;340(2):409-16.
REF 32 Raf-1 promotes cell survival by antagonizing apoptosis signal-regulating kinase 1 through a MEK-ERK independent mechanism. Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7783-8.
REF 33 The caspase-8 inhibitor FLIP promotes activation of NF-kappaB and Erk signaling pathways. Curr Biol. 2000 Jun 1;10(11):640-8.
REF 34 Toward an understanding of the protein interaction network of the human liver. Mol Syst Biol. 2011 Oct 11;7:536.
REF 35 14-3-3 isotypes facilitate coupling of protein kinase C-zeta to Raf-1: negative regulation by 14-3-3 phosphorylation. Biochem J. 2000 Jan 15;345 Pt 2:297-306.
REF 36 Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis. Genomics. 2003 Feb;81(2):112-25.
REF 37 14-3-3 proteins associate with A20 in an isoform-specific manner and function both as chaperone and adapter molecules. J Biol Chem. 1996 Aug 16;271(33):20029-34.
REF 38 14-3-3 proteins associate with cdc25 phosphatases. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7892-6.
REF 39 Prohibitin is required for Ras-induced Raf-MEK-ERK activation and epithelial cell migration. Nat Cell Biol. 2005 Aug;7(8):837-43.
REF 40 Enabling systematic interrogation of protein-protein interactions in live cells with a versatile ultra-high-throughput biosensor platform. J Mol Cell Biol. 2016 Jun;8(3):271-81.
REF 41 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 42 Large-scale identification of c-MYC-associated proteins using a combined TAP/MudPIT approach. Cell Cycle. 2007 Jan 15;6(2):205-17.
REF 43 c-Raf kinase binds to N-terminal domain of c-Myc. FEBS Lett. 1997 Sep 8;414(2):465-70.
REF 44 15-Lipoxygenase 1 interacts with phosphatidylethanolamine-binding protein to regulate MAPK signaling in human airway epithelial cells. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14246-51.
REF 45 Rb and prohibitin target distinct regions of E2F1 for repression and respond to different upstream signals. Mol Cell Biol. 1999 Nov;19(11):7447-60.
REF 46 RanBPM is an inhibitor of ERK signaling. PLoS One. 2012;7(10):e47803.
REF 47 Epithelial sodium channel regulated by differential composition of a signaling complex. Proc Natl Acad Sci U S A. 2009 May 12;106(19):7804-9.
REF 48 Glucocorticoid-induced leucine zipper inhibits the Raf-extracellular signal-regulated kinase pathway by binding to Raf-1. Mol Cell Biol. 2002 Nov;22(22):7929-41.

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