Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T55244 Target Info
Target Name Gamma-Histone H2AX (H2AFX)
Synonyms Phosphorylation of H2AX; Histone H2AX; Histone H2A.x; H2a/x; H2AX
Target Type Literature-reported Target
Gene Name H2AFX
UniProt ID
H2AX_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 3 Acid anhydride hydrolases +
Interacting Protein Name X-ray repair cross-complementing protein 6 (XRCC6) Interacting Protein Interacting Protein Info
UniProt ID
XRCC6_HUMAN 
Synonyms CTC75; CTCBF; G22P1; KU70; ML8; TLAA
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [1]
5 Affinity Capture-Western [4]
6 Reconstituted Complex [1]
7 Reconstituted Complex [5]
Interacting Target Name Mitotic growth and transcription activator (BAF190A) Literature-reported Target Interacting Protein Info
UniProt ID
SMCA4_HUMAN 
Synonyms Transcription activator BRG1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SNF2L4; SNF2-beta; SNF2B; Protein BRG-1; Protein brahma homolog 1; BRG1-associated factor 190A; BRG1; ATP-dependent helicase SMARCA4
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [6]
2 Affinity Capture-Western [4]
3 Reconstituted Complex [4]
Interacting Protein Name DNA repair protein RAD50 (RAD50) Interacting Protein Interacting Protein Info
UniProt ID
RAD50_HUMAN 
Synonyms NBSLD; RAD502; hRad50
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Co-localization [8]
Acyltransferases [+] 2 Acyltransferases +
Interacting Protein Name Histone acetyltransferase KAT5 (KAT5) Interacting Protein Interacting Protein Info
UniProt ID
KAT5_HUMAN 
Synonyms ESA1; HTATIP; HTATIP1; PLIP; TIP; TIP60; ZC2HC5; cPLA2
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [1]
3 Affinity Capture-Western [4]
4 Reconstituted Complex [1]
Interacting Protein Name Breast cancer type 1 susceptibility protein (BRCA1) Interacting Protein Interacting Protein Info
UniProt ID
BRCA1_HUMAN 
Synonyms BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Affinity Capture-Western [10]
3 Co-localization [8]
Ester hydrolases [+] 3 Ester hydrolases +
Interacting Protein Name Double-strand break repair protein MRE11 (MRE11) Interacting Protein Interacting Protein Info
UniProt ID
MRE11_HUMAN 
Synonyms ATLD; HNGS1; MRE11; MRE11B
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Affinity Capture-Western [11]
3 Co-localization [11]
Interacting Protein Name Protein phosphatase 1G (PPM1G) Interacting Protein Interacting Protein Info
UniProt ID
PPM1G_HUMAN 
Synonyms PP2CG; PP2CGAMMA; PPP2CG
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-Western [1]
Interacting Target Name Protein phosphatase 1D (PPM1D) Literature-reported Target Interacting Protein Info
UniProt ID
PPM1D_HUMAN 
Synonyms WIP1; Protein phosphatase magnesium-dependent 1 delta; Protein phosphatase 2C isoform delta; PP2C-delta; p53-induced protein phosphatase 1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Biochemical Activity [13]
Histones [+] 2 Histones +
Interacting Protein Name Histone H2B type 2-E (HIST2H2BE) Interacting Protein Interacting Protein Info
UniProt ID
H2B2E_HUMAN 
Synonyms GL105; H2B; H2B.1; H2BFQ; H2BGL105; H2BQ
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [14]
3 Affinity Capture-Western [4]
Interacting Protein Name Histone H4 (H4) Interacting Protein Interacting Protein Info
UniProt ID
H4_HUMAN 
Synonyms H4FA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-MS [15]
3 Affinity Capture-Western [4]
Kinases [+] 4 Kinases +
Interacting Target Name Casein kinase II alpha (CSNK2A1) Clinical trial Target Interacting Protein Info
UniProt ID
CSK21_HUMAN 
Synonyms Protein kinase CK2; CK2A1; CK II alpha; CK II; Casein kinase II subunit alpha
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [16]
2 Co-localization [17]
Interacting Target Name Stress-activated protein kinase JNK1 (JNK1) Clinical trial Target Interacting Protein Info
UniProt ID
MK08_HUMAN 
Synonyms Stress-activated protein kinase 1c; SAPK1c; SAPK1; PRKM8; Mitogen-activated protein kinase 8; MAPK 8; MAP kinase 8; JNK-46; C-Jun N-terminal kinase 1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [18]
2 Reconstituted Complex [19]
Interacting Target Name ATM serine/threonine kinase (ATM) Clinical trial Target Interacting Protein Info
UniProt ID
ATM_HUMAN 
Synonyms Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Biochemical Activity [20]
Interacting Target Name Serine/threonine-protein kinase ATR (FRP1) Clinical trial Target Interacting Protein Info
UniProt ID
ATR_HUMAN 
Synonyms FRAP-related protein 1; Ataxia telangiectasia and Rad3-related protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [20]
2 Co-localization [9]
Peptidases [+] 1 Peptidases +
Interacting Protein Name FACT complex subunit SPT16 (SUPT16H) Interacting Protein Interacting Protein Info
UniProt ID
SP16H_HUMAN 
Synonyms CDC68; FACTP140; SPT16/CDC68
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [1]
4 Affinity Capture-Western [1]
5 Reconstituted Complex [1]
Ribosomal proteins [+] 1 Ribosomal proteins +
Interacting Protein Name Small ribosomal subunit protein Es10 (RPS10) Interacting Protein Interacting Protein Info
UniProt ID
RS10_HUMAN 
Synonyms DBA9; S10
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [15]
2 Affinity Capture-MS [2]
Other proteins [+] 10 Other proteins +
Interacting Target Name Poly [ADP-ribose] polymerase 1 (PARP1) Successful Target Interacting Protein Info
UniProt ID
PARP1_HUMAN 
Synonyms Protein poly-ADP-ribosyltransferase PARP1; PPOL; Poly[ADP-ribose] synthetase-1; Poly[ADP-ribose] synthase 1; Poly(ADP-ribose)polymerase-1; PARP-1; NAD(+)Poly [ADP-ribose] polymerase-1 ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase 1; DNA ADP-ribosyltransferase PARP1; ARTD1; ADPRT 1; ADPRT; ADP-ribosyltransferase diphtheria toxin-like 1
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [15]
5 Affinity Capture-Western [14]
6 Affinity Capture-Western [1]
7 Affinity Capture-Western [15]
8 Reconstituted Complex [1]
Interacting Protein Name Nibrin (NBN) Interacting Protein Interacting Protein Info
UniProt ID
NBN_HUMAN 
Synonyms AT.V1; AT.V2; ATV; NBS; NBS1; P95
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-Western [6]
2 Affinity Capture-Western [21]
3 Affinity Capture-Western [7]
4 Affinity Capture-Western [11]
5 Reconstituted Complex [22]
6 Reconstituted Complex [11]
7 Reconstituted Complex [5]
Interacting Protein Name Mediator of DNA damage checkpoint protein 1 (MDC1) Interacting Protein Interacting Protein Info
UniProt ID
MDC1_HUMAN 
Synonyms NFBD1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Affinity Capture-Western [23]
3 Affinity Capture-Western [21]
4 Co-localization [23]
5 Reconstituted Complex [23]
Interacting Target Name Calreticulin (CALR) Literature-reported Target Interacting Protein Info
UniProt ID
CALR_HUMAN 
Synonyms HACBP; grp60; ERp60; ERC; Endoplasmic reticulum resident protein 60; CRTC; CRP55; Calregulin
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-MS [15]
3 Affinity Capture-Western [14]
4 Affinity Capture-Western [15]
Interacting Target Name Nucleophosmin (NPM1) Clinical trial Target Interacting Protein Info
UniProt ID
NPM_HUMAN 
Synonyms Numatrin; Nucleolar protein NO38; Nucleolar phosphoprotein B23; NPM
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-MS [2]
3 Affinity Capture-Western [14]
Interacting Target Name Facilitates chromatin transcription complex (FACT) Clinical trial Target Interacting Protein Info
UniProt ID
SSRP1_HUMAN 
Synonyms T160; Structure-specific recognition protein 1; Recombination signal sequence recognition protein 1; hSSRP1; FACTp80; FACT80; FACT complex subunit SSRP1; FACT 80 kDa subunit; Facilitates chromatin transcription complex subunit SSRP1; Facilitates chromatin transcription complex 80 kDa subunit; Chromatin-specific transcription elongation factor 80 kDa subunit
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-MS [2]
3 Reconstituted Complex [1]
Interacting Target Name Polycomb complex protein BMI-1 (BMI1) Clinical trial Target Interacting Protein Info
UniProt ID
BMI1_HUMAN 
Synonyms RNF51; RING finger protein 51; Polycomb group RING finger protein 4; PCGF4
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [21]
2 Affinity Capture-Western [21]
3 Co-localization [24]
Interacting Target Name Breast cancer type 2 susceptibility protein (BRCA2) Literature-reported Target Interacting Protein Info
UniProt ID
BRCA2_HUMAN 
Synonyms Fanconi anemia group D1 protein; FANCD1; FACD
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [1]
3 Reconstituted Complex [25]
Interacting Target Name Tumor suppressor p53-binding protein 1 (TP53BP1) Literature-reported Target Interacting Protein Info
UniProt ID
TP53B_HUMAN 
Synonyms TP53-binding protein 1; p53BP1; p53-binding protein 1; 53BP1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [23]
2 Co-localization [26]
Interacting Protein Name Interleukin enhancer-binding factor 2 (ILF2) Interacting Protein Interacting Protein Info
UniProt ID
ILF2_HUMAN 
Synonyms NF45; PRO3063
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [15]
2 Affinity Capture-MS [2]
References  Top
REF 1 FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Mol Cell. 2008 Apr 11;30(1):86-97.
REF 2 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 3 Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II. J Biol Chem. 2005 Mar 11;280(10):9586-94.
REF 4 A cooperative activation loop among SWI/SNF, gamma-H2AX and H3 acetylation for DNA double-strand break repair. EMBO J. 2010 Apr 21;29(8):1434-45.
REF 5 Nucleolin participates in DNA double-strand break-induced damage response through MDC1-dependent pathway. PLoS One. 2012;7(11):e49245.
REF 6 Mammalian SWI/SNF complexes facilitate DNA double-strand break repair by promoting gamma-H2AX induction. EMBO J. 2006 Sep 6;25(17):3986-97.
REF 7 Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention. EMBO J. 2004 Jul 7;23(13):2674-83.
REF 8 A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol. 2000 Jul 27-Aug 10;10(15):886-95.
REF 9 Human SNF5/INI1, a component of the human SWI/SNF chromatin remodeling complex, promotes nucleotide excision repair by influencing ATM recruitment and downstream H2AX phosphorylation. Mol Cell Biol. 2009 Dec;29(23):6206-19.
REF 10 Functional interaction between FOXO3a and ATM regulates DNA damage response. Nat Cell Biol. 2008 Apr;10(4):460-7.
REF 11 NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain. Curr Biol. 2002 Oct 29;12(21):1846-51.
REF 12 Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. J Biol Chem. 2010 Apr 23;285(17):12935-47.
REF 13 Wip1 phosphatase is associated with chromatin and dephosphorylates gammaH2AX to promote checkpoint inhibition. Oncogene. 2010 Apr 15;29(15):2281-91.
REF 14 The dynamic alterations of H2AX complex during DNA repair detected by a proteomic approach reveal the critical roles of Ca(2+)/calmodulin in the ionizing radiation-induced cell cycle arrest. Mol Cell Proteomics. 2006 Jun;5(6):1033-44.
REF 15 Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma. J Proteome Res. 2010 Mar 5;9(3):1402-15.
REF 16 Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation. Nature. 2014 Dec 11;516(7530):267-71.
REF 17 Protein kinase CK2 localizes to sites of DNA double-strand break regulating the cellular response to DNA damage. BMC Mol Biol. 2012 Mar 9;13:7.
REF 18 DNA damage during mitosis invokes a JNK-mediated stress response that leads to cell death. J Cell Biochem. 2010 Jun 1;110(3):725-31.
REF 19 Lymphokine-activated killer T-cell-originated protein kinase phosphorylation of histone H2AX prevents arsenite-induced apoptosis in RPMI7951 melanoma cells. Clin Cancer Res. 2006 Dec 1;12(23):6884-93.
REF 20 Vpr-binding protein antagonizes p53-mediated transcription via direct interaction with H3 tail. Mol Cell Biol. 2012 Feb;32(4):783-96.
REF 21 Monoubiquitination of H2AX protein regulates DNA damage response signaling. J Biol Chem. 2011 Aug 12;286(32):28599-607.
REF 22 Werner syndrome protein associates with gamma H2AX in a manner that depends upon Nbs1. FEBS Lett. 2005 Feb 28;579(6):1350-6.
REF 23 MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature. 2003 Feb 27;421(6926):961-6.
REF 24 BMI1 is recruited to DNA breaks and contributes to DNA damage-induced H2A ubiquitination and repair. Mol Cell Biol. 2011 May;31(10):1972-82.
REF 25 BRCA1 Forms a Functional Complex with -H2AX as a Late Response to Genotoxic Stress. J Nucleic Acids. 2010 Sep 27;2010. pii: 801594.
REF 26 Evidence for ongoing DNA damage in multiple myeloma cells as revealed by constitutive phosphorylation of H2AX. Leukemia. 2011 Aug;25(8):1344-53.

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