Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T62449 Target Info
Target Name Checkpoint kinase-1 (CHK1)
Synonyms Serine/threonine-protein kinase Chk1; Chk1; Cell cycle checkpoint kinase; CHK1 checkpoint homolog
Target Type Clinical trial Target
Gene Name CHEK1
Biochemical Class Kinase
UniProt ID
CHK1_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 1 Acyltransferases +
Interacting Protein Name Breast cancer type 1 susceptibility protein (BRCA1) Interacting Protein Interacting Protein Info
UniProt ID
BRCA1_HUMAN 
Synonyms BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Biochemical Activity [2]
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Target Name M-phase inducer phosphatase 1 (MPIP1) Literature-reported Target Interacting Protein Info
UniProt ID
MPIP1_HUMAN 
Synonyms Dual specificity phosphatase Cdc25A; Cdc25A phosphatase
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Biochemical Activity [4]
3 Biochemical Activity [5]
4 Biochemical Activity [6]
5 Biochemical Activity [7]
6 Biochemical Activity [8]
7 Reconstituted Complex [9]
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Target Name Heat shock protein 90 alpha (HSP90A) Successful Target Interacting Protein Info
UniProt ID
HS90A_HUMAN 
Synonyms Renal carcinoma antigen NY-REN-38; LPS-associated protein 2; Lipopolysaccharide-associated protein 2; LAP-2; HSPCA; HSPC1; HSP90A; HSP86; HSP 86; Heat shock protein HSP 90-alpha; Heat shock 86 kDa
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Luminescence [10]
2 Affinity Capture-Western [11]
Kinases [+] 2 Kinases +
Interacting Target Name Serine/threonine-protein kinase ATR (FRP1) Clinical trial Target Interacting Protein Info
UniProt ID
ATR_HUMAN 
Synonyms FRAP-related protein 1; Ataxia telangiectasia and Rad3-related protein
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Biochemical Activity [13]
3 Protein-peptide [14]
Interacting Target Name ATM serine/threonine kinase (ATM) Clinical trial Target Interacting Protein Info
UniProt ID
ATM_HUMAN 
Synonyms Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [5]
2 Protein-peptide [14]
Peptidases [+] 1 Peptidases +
Interacting Target Name Herpesvirus ubiquitin-specific protease (HAUSP) Preclinical Target Interacting Protein Info
UniProt ID
UBP7_HUMAN 
Synonyms Ubiquitin-specific-processing protease 7; Ubiquitin thioesterase 7; Ubiquitin carboxyl-terminal hydrolase 7; Herpesvirus-associated ubiquitin-specific protease; Deubiquitinating enzyme 7
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [15]
2 Biochemical Activity [16]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [17]
2 Biochemical Activity [18]
3 Biochemical Activity [19]
4 Co-localization [20]
Other proteins [+] 2 Other proteins +
Interacting Protein Name Claspin (CLSPN) Interacting Protein Interacting Protein Info
UniProt ID
CLSPN_HUMAN 
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [21]
2 Affinity Capture-Western [22]
3 Affinity Capture-Western [23]
4 Biochemical Activity [24]
5 Reconstituted Complex [21]
6 Reconstituted Complex [24]
Interacting Protein Name DNA damage-binding protein 1 (DDB1) Interacting Protein Interacting Protein Info
UniProt ID
DDB1_HUMAN 
Synonyms DDBA; UV.DDB1; XAP1; XPCE; XPE; XPE.BF
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-Western [25]
3 Affinity Capture-Western [22]
4 Affinity Capture-Western [26]
5 Affinity Capture-Western [27]
References  Top
REF 1 BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage. Nat Genet. 2002 Mar;30(3):285-9.
REF 2 Phosphorylation of pRB at Ser612 by Chk1/2 leads to a complex between pRB and E2F-1 after DNA damage. EMBO J. 2007 Apr 18;26(8):2083-93.
REF 3 Defining the Protein-Protein Interaction Network of the Human Protein Tyrosine Phosphatase Family. Mol Cell Proteomics. 2016 Sep;15(9):3030-44.
REF 4 Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science. 1997 Sep 5;277(5331):1497-501.
REF 5 Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A. Cancer Cell. 2003 Mar;3(3):247-58.
REF 6 Disruption of the checkpoint kinase 1/cell division cycle 25A pathway abrogates ionizing radiation-induced S and G2 checkpoints. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14795-800.
REF 7 Differential roles for checkpoint kinases in DNA damage-dependent degradation of the Cdc25A protein phosphatase. J Biol Chem. 2008 Jul 11;283(28):19322-8.
REF 8 Phosphorylation at serine 75 is required for UV-mediated degradation of human Cdc25A phosphatase at the S-phase checkpoint. J Biol Chem. 2003 Aug 8;278(32):29824-9.
REF 9 Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint. Cell Cycle. 2003 Sep-Oct;2(5):473-8.
REF 10 Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. Cell. 2012 Aug 31;150(5):987-1001.
REF 11 Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage. Mol Cancer Ther. 2011 Jul;10(7):1194-206.
REF 12 Regulation of NF-kappaB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor. EMBO J. 2005 Mar 23;24(6):1157-69.
REF 13 Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress. Oncogene. 2009 May 7;28(18):1971-81.
REF 14 Substrate specificities and identification of putative substrates of ATM kinase family members. J Biol Chem. 1999 Dec 31;274(53):37538-43.
REF 15 USP7 regulates the stability and function of HLTF through deubiquitination. J Cell Biochem. 2011 Dec;112(12):3856-62.
REF 16 ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1. Nat Cell Biol. 2014 Sep;16(9):864-75.
REF 17 Radiation-induced phosphorylation of Chk1 at S345 is associated with p53-dependent cell cycle arrest pathways. Neoplasia. 2002 Mar-Apr;4(2):171-80.
REF 18 Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex. J Biol Chem. 2003 Aug 8;278(32):29940-7.
REF 19 The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites. Genes Dev. 2000 Feb 1;14(3):289-300.
REF 20 Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest. J Cell Biol. 2004 Sep 13;166(6):801-13.
REF 21 Human claspin is required for replication checkpoint control. J Biol Chem. 2003 Aug 8;278(32):30057-62.
REF 22 CRL4(CDT2) targets CHK1 for PCNA-independent destruction. Mol Cell Biol. 2013 Jan;33(2):213-26.
REF 23 Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway. J Biol Chem. 2005 Oct 21;280(42):35337-45.
REF 24 Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function. J Biol Chem. 2006 Nov 3;281(44):33276-82.
REF 25 DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress. Cancer Res. 2009 Mar 15;69(6):2630-7.
REF 26 The DNA repair component Metnase regulates Chk1 stability. Cell Div. 2014 Jul 9;9:1.
REF 27 TTF-1/NKX2-1 binds to DDB1 and confers replication stress resistance to lung adenocarcinomas. Oncogene. 2017 Jun 29;36(26):3740-3748.

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